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Det Bästa Lgr5 - Ruz In Cunningham

Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5 tm1(cre/ESR1)Cle: Gene: Lgr5 Location: Chr10:115450314-115587780 bp, - strand Genetic Position: Chr10, 63.51 cM In Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice, canonical Wnt signaling was potently induced by systemic administration of an adenovirus encoding Rspo1 fused to an IgG2α Fc fragment (Ad Rspo1-Fc), causing marked crypt hypertrophy and hyperproliferation. By 5 d after infection, Ad Rspo1-Fc markedly expanded Lgr5-eGFP+ The Jackson Laboratory lgr5 egfp ires creert2 Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Inhibition of BMP signaling in Lgr5 cells was achieved by crossing Lgr5-EGFP-ires-CreERT2 (Lgr5-Cre) mice to mice with floxed alleles of BMP receptor 1A (Lgr5-Cre;Bmpr1aflox/flox mice). Lgr5/GFP+ve cells were isolated using flow cytometry. Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were used to compare the basal proliferation status of Lgr5 + vs.

Lgr5-egfp-ires-creert2

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Lineage tracing in the small intestine and colon.a, Lgr5-EGFP-IRES-creERT2 knock-in mouse crossed with Rosa26-lacZ reporter mice 12 h after tamoxifen injection. b, Frequency at which the blue Se hela listan på genoway.com ApcMin and Lgr5-EGFP-ires-CreERT2 mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Foxf1 and Foxf2 targeted mutants have been described elsewhere.22,23 Tg(FOXF2) was generated by pronuclear injection of a linearized BAC (RP4-668J24) spanning 120,429 base pairs of the human FOXF2 locus. 2020-06-10 · (n = 1 with 3 replicates for WT, all C3(1)-Tag, and healthy MMTV-PyMT; n = 2 MMTV-PyMT for Lgr4, Lgr6, and Lgr5 carcinoma, n = 4 MMTV-PyMT for Lgr5 hyperplasia and adenoma) b Tamoxifen (Tam)-induced Cre recombinase knocked into the Lgr5 locus, Lgr5-EGFP-IRES-creERT2 crossed to Tomato reporter mice, Ai14(R26R dtTomato) indicated Lgr5 + cell progeny in hyperplasias (whole mounts, 1-week Tam Lgr5EGFP-Ires-CreERT2/Apc fl/flmice with the R26R-Confetti strain. Upon stochastic Cre induction by low-dose tamoxifen in adult mice, intestinal ade-nomas developed from Apc-deleted Lgr5+ stem cells.After4weeks,weobservedlargeadenomas (fig. S2, A to C) that either uniformly expressed a single Confetti colororconsistedofanatomically To this end, we first established a sorting strategy that would enable the isolation of pure populations of Lgr5 + E10.5-E12.5 hepatoblasts using the Lgr5-EGFP-IRES-creERT2 mouse line, where the eGFP reporter is knocked-in into the Lgr5 locus (Barker et al., 2007), combined with co-staining using an anti-Liv2 antibody, which specifically labels E9.5-E13.5 liver progenitors (Nierhoff et al 2021-01-15 · Lgr5-eGFP-IRES-CreERT2 mice were crossed with B6.Cg-Gt (ROSA)26Sortm9(CAG-tdTomato)Hze/J mice (Jackson Laboratories) to generate Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato heterozygote.

Gastriska lgr5 + stamceller är det cellulära ursprunget till invasiv

Expression of Bmi1, another putative N et al. Biol Res P 6 13 G007‑LKeatment repressed lineage tracing from˜ LGR5+ intestinal stem cells Lgr5-EGFP-Ires-CreERT2;R26R-Confettitdoublettrans- genictmicet adult Lgr5-EGFP-ires-CreERT2/Rosa four-color mice.

Lgr5-egfp-ires-creert2

Stamceller i tarmscancer präglade av uttrycket bmi1 eller lgr5 bidrar

In contrast, only 1.7 ± 0.30% of Bmi1+ ISCs, identified by the crypt Rosa-YFP+ signal after 1.5-d tamoxifen exposure in Bmi1-CreER; Rosa26-YFP mice, incorporated EdU (Fig. 1 A–F and N). To examine the relative contribution of Lgr5 vs.

Lgr5-egfp-ires-creert2

(A) Apc-deficient adenomas were allowed to develop for 24 days in Lgr5-EGFP-Ires-CreERT2/Apcfl/fl/R26R  Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function  were generated by crossing Lgr5–EGFP–ires–CreERT2 and. Klf5 flox/flox mice ( 4, 29). Because Cre–ERT2 fusion gene activity is inducible following tamoxifen  In 4 Lgr5-EGFP-IRES-CreERT2 Kras LSL-G12D mice studied between 2 and 8 months after tamoxifen. Figure 1. Hyperplastic and hyperproliferation changes in   Notch Signaling Regulates Gastric Antral LGR5 Stem Cell Function. Elise S. Demitrack1 Lgr5-EGFP-IRES-CreERT2; ROSA-LSL-Confetti; ROSANotchIC.
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Neonate (P1) Lgr5-EGFP-ires-CreERT2/R26RLacZ mice were given a single 100 μg dose of 4-hydroxytamoxifen to activate the Cre enzyme and, as a consequence, permanently switch on the lacZ reporter gene within renal Lgr5 +ve cells. View Lgr5 allele: origin, molecular description, gene associations, and references. To delete exons 2–10 of Trp53 in colonic stem cells, Trp53 flox/flox mice (Jackson, 008462) or Rosa26-LacZ mice (Jackson, 003474) were crossed with Lgr5-EGFP-IRES-creERT2 mice (Jackson, 008875; ref.

南模生物. 利用CRISPR基因编辑技术，将EGFP-IRES-creERT2-WPRE-polyA插入到小鼠Lgr5基因起始密码子处。 Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were treated with 12 Gy whole-body irradiation. By 2 d after irradiation, Lgr5-eGFP + ISCs and Olfm4 expression were completely lost from small intestine crypts ( Fig. 2 A , C , I – K , and L ), whereas there were no discernible quantitative effects on Bmi1-YFP + ISCs labeled with 1-d tamoxifen treatment ( Fig. 2 B and D ).
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Gastriska lgr5 + stamceller är det cellulära ursprunget till invasiv

3p) undersöktes för EGFP-uttryck som indikerar Lgr5 +  While homozygous mice are not viable, heterozygous Lgr5-EGFP-IRES-CreERT2 mice are viable and fertile; harboring a Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements. A EGFP-IRES-creERT2-WPRE-polyA expression cassette was knocked into the Lgr5 gene start codon site via CRISPR/Cas9 mediated recombination. Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements.


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Inaktivering av tgfp-receptorer i stamceller driver kutant

Swiss roll sections of paraffin-embedded intestines were prepared and immunohistochemically analyzed. May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-. creERT2 (43). Mice treated with tamoxifen and monitored for 4,. 5, or 7 d showed a  In sharp contrast to control SPA-Cre; Cdh1fl/+; Lgr5-eGFP-IRES-CreERT2 mice, where GFP- positive Lgr5+ cells resided at the base of the glands in both the  Lgr5-EGFP-IRES-CreERT2/Rosa-LSL-tdTomato mice.

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Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques.

May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-. creERT2 (43). Mice treated with tamoxifen and monitored for 4,.